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1. Sample raw data: enclosed is a text file with dataset download links.
2. File types for inputs and outputs at each step (particularly for FASTQC and
TrimGalore (first two steps) operations)
Enclosed as a table below (also enclosed the corresponding excel file):
S.No. Analysis stage Tool used Input file type Code Output data type 1 Quality check FastQC fq / fastq ./fastqc
*fastq.gz text, html QC reports 2 Quality trimming TrimGalore fq / fastq
trim_galore --gzip --fastqc --max_n 2 --paired --length 50
SRR11862696_1.fastq.gz SRR11862696_2.fastq.gz fq; fastq